Gram staining protocol ppt
Gram Staining Procedure/Protocol: Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent. Please note that the quality of the smear (too heavy or too light cell concentration) will affect the Gram Stain results. Wash slide in a gentle and indirect stream of tap water for 2 seconds.
If the Gram staining procedure is done correctly, your slide should have a mixture of gram-negative and gram-positive cells as well as some neutrophils (white blood cells) with pink nuclei. After you have tried that, stain each of your live bacteria cultures using the following procedure: Oct 18, 2016 · • Mainly used differential staining is GRAM’S STAINING 10 11. 11 12. HANS CHRISTIAN GRAM 12 13. HANS CHRISTIAN GRAM INTRODUCED GRAM’S STAINING 13 14. GRAM’S STAINING • Gram Stain is developed in 1884 by the Danish physician Christian Gram, is the most widely used method in bacteriology. Gram Stain Protocol pt.5 Flood the slide with safranin for 1 minute and rinse all excess dye off with dI water. Dry your slide with bibulous paper. Your stain is now ready to be microscopically examined. The Gram stain is performed on a sediment of the CSF and is positive in at least 60% of cases of bacterial meningitis. Culture is performed for both aerobic and anaerobic bacteria. In addition, other stains (e.g. the acid-fast stain for Mycobacterium tuberculosis , fungal culture, and rapid identification tests [tests for bacterial and fungal ... Gram Stain Protocol This technique is used to stain a slide such as a fecal smear to observe the bacterial microflora present based on their gram stain reaction. "Heat-fix" the slide with the specimen by passing it over a heat source, such as a flame, several times using a clothes pin or forceps. The Gram Stain Kit (Microorganism Stain) is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria. Other products for staining tissue sections Find more kits and reagents in the special stains guide , or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide .
In 1884, Hans Christian Gram, a Danish doctor, developed a differential staining technique that is still the cornerstone of bacterial identification and taxonomic division. This multistep, sequential staining protocol separates bacteria into four groups based on cell morphology and cell wall structure: Gram‐positive cocci, Gram‐negative ...
Gram Staining Kit is intended for use as a qualitative histologic stain to demonstrate gram-negative and gram-positive bacteria in formalin-fixed, paraffin-embedded tissue. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. Dark fungi suspected by routine staining procedures must be confirmed by special melanin stain known as Fontana Masson (FM). In the image FM x1000 morphology of the fungal elements remains similar to Gram stain. The cell wall is darker, confirms dematiaceous fungus involved.
SMEAR PREPARATION. The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure.